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Literature summary extracted from
- Extended recognition of the histone H3 tail by histone demethylase KDM5A (2020), Biochemistry, 59, 647-651 .
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.14.11.67 | 0.009 | - |
[histone H3 N-terminal 21mer mutant T3A] N6,N6,N6-trimethyl-L-lysine4 | pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
| 1.14.11.67 | 0.0125 | - |
[histone H3 N-terminal 21mer] N6,N6,N6-trimethyl-L-lysine4 | pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
| 1.14.11.67 | 0.0147 | - |
[histone H3 N-terminal 21mer mutant T6S] N6,N6,N6-trimethyl-L-lysine4 | pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
| 1.14.11.67 | 0.0219 | - |
[histone H3 N-terminal 21mer mutant K9A] N6,N6,N6-trimethyl-L-lysine4 | pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
| 1.14.11.67 | 0.0394 | - |
[histone H3 N-terminal 21mer mutant T6V] N6,N6,N6-trimethyl-L-lysine4 | pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
| 1.14.11.67 | 0.0551 | - |
[histone H3 N-terminal 21mer mutant R8A] N6,N6,N6-trimethyl-L-lysine4 | pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
| 1.14.11.67 | 0.0623 | - |
[histone H3 N-terminal 18mer] N6,N6,N6-trimethyl-L-lysine4 | pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
| 1.14.11.67 | 0.0965 | - |
[histone H3 N-terminal 21mer mutant T6A] N6,N6,N6-trimethyl-L-lysine4 | pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
| 1.14.11.67 | 0.1536 | - |
[histone H3 N-terminal 21mer mutant R2A] N6,N6,N6-trimethyl-L-lysine4 | pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
| 1.14.11.67 | 0.249 | - |
[histone H3 N-terminal 18mer mutant K14ac/K18ac] N6,N6,N6-trimethyl-L-lysine4 | pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
| 1.14.11.67 | 0.4152 | - |
[histone H3 N-terminal 21mer mutant Q5A] N6,N6,N6-trimethyl-L-lysine4 | pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
| 1.14.11.67 | 0.489 | - |
[histone H3 N-terminal 13mer] N6,N6,N6-trimethyl-L-lysine4 | pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
| 1.14.11.67 | 0.514 | - |
[histone H3 N-terminal 18mer mutant K14A/R17A/K18A] N6,N6,N6-trimethyl-L-lysine4 | pH not specified in the publication, temperature not specified in the publication | Homo sapiens |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.14.11.67 | Homo sapiens | P29375 | - |
- |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.11.67 | [histone H3 N-terminal 13mer] N6,N6,N6-trimethyl-L-lysine4 + 2-oxoglutarate + O2 | H3 residue Q5 is critical for substrate recognition by KDM5A | Homo sapiens | [histone H3 N-terminal 13mer] N6,N6-dimethyl-L-lysine4 + succinate + formaldehyde + CO2 | - |
? |  |
| 1.14.11.67 | [histone H3 N-terminal 18mer mutant K14A/R17A/K18A] N6,N6,N6-trimethyl-L-lysine4 + 2-oxoglutarate + O2 | H3 residue Q5 is critical for substrate recognition by KDM5A | Homo sapiens | [histone H3 N-terminal 18mer mutant K14A/R17A/K18A] N6,N6-dimethyl-L-lysine4 + succinate + formaldehyde + CO2 | - |
? | |
| 1.14.11.67 | [histone H3 N-terminal 18mer mutant K14ac/K18ac] N6,N6,N6-trimethyl-L-lysine4 + 2-oxoglutarate + O2 | H3 residue Q5 is critical for substrate recognition by KDM5A | Homo sapiens | [histone H3 N-terminal 18mer mutant K14ac/K18ac] N6,N6-dimethyl-L-lysine4 + succinate + formaldehyde + CO2 | - |
? | |
| 1.14.11.67 | [histone H3 N-terminal 18mer] N6,N6,N6-trimethyl-L-lysine4 + 2-oxoglutarate + O2 | H3 residue Q5 is critical for substrate recognition by KDM5A | Homo sapiens | [histone H3 N-terminal 18mer] N6,N6-dimethyl-L-lysine4 + succinate + formaldehyde + CO2 | - |
? | |
| 1.14.11.67 | [histone H3 N-terminal 21mer mutant K9A] N6,N6,N6-trimethyl-L-lysine4 + 2-oxoglutarate + O2 | H3 residue Q5 is critical for substrate recognition by KDM5A | Homo sapiens | [histone H3 N-terminal 21mer mutant K9A] N6,N6-dimethyl-L-lysine4 + succinate + formaldehyde + CO2 | - |
? | |
| 1.14.11.67 | [histone H3 N-terminal 21mer mutant Q5A] N6,N6,N6-trimethyl-L-lysine4 + 2-oxoglutarate + O2 | H3 residue Q5 is critical for substrate recognition by KDM5A | Homo sapiens | [histone H3 N-terminal 21mer mutant Q5A] N6,N6-dimethyl-L-lysine4 + succinate + formaldehyde + CO2 | - |
? | |
| 1.14.11.67 | [histone H3 N-terminal 21mer mutant R2A] N6,N6,N6-trimethyl-L-lysine4 + 2-oxoglutarate + O2 | H3 residue Q5 is critical for substrate recognition by KDM5A | Homo sapiens | [histone H3 N-terminal 21mer mutant R2A] N6,N6-dimethyl-L-lysine4 + succinate + formaldehyde + CO2 | - |
? | |
| 1.14.11.67 | [histone H3 N-terminal 21mer mutant R8A] N6,N6,N6-trimethyl-L-lysine4 + 2-oxoglutarate + O2 | H3 residue Q5 is critical for substrate recognition by KDM5A | Homo sapiens | [histone H3 N-terminal 21mer mutant R8A] N6,N6-dimethyl-L-lysine4 + succinate + formaldehyde + CO2 | - |
? | |
| 1.14.11.67 | [histone H3 N-terminal 21mer mutant T3A] N6,N6,N6-trimethyl-L-lysine4 + 2-oxoglutarate + O2 | H3 residue Q5 is critical for substrate recognition by KDM5A | Homo sapiens | [histone H3 N-terminal 21mer mutant T3A] N6,N6-dimethyl-L-lysine4 + succinate + formaldehyde + CO2 | - |
? | |
| 1.14.11.67 | [histone H3 N-terminal 21mer mutant T6A] N6,N6,N6-trimethyl-L-lysine4 + 2-oxoglutarate + O2 | H3 residue Q5 is critical for substrate recognition by KDM5A | Homo sapiens | [histone H3 N-terminal 21mer mutant T6A] N6,N6-dimethyl-L-lysine4 + succinate + formaldehyde + CO2 | - |
? | |
| 1.14.11.67 | [histone H3 N-terminal 21mer mutant T6S] N6,N6,N6-trimethyl-L-lysine4 + 2-oxoglutarate + O2 | H3 residue Q5 is critical for substrate recognition by KDM5A | Homo sapiens | [histone H3 N-terminal 21mer mutant T6S] N6,N6-dimethyl-L-lysine4 + succinate + formaldehyde + CO2 | - |
? | |
| 1.14.11.67 | [histone H3 N-terminal 21mer mutant T6V] N6,N6,N6-trimethyl-L-lysine4 + 2-oxoglutarate + O2 | H3 residue Q5 is critical for substrate recognition by KDM5A | Homo sapiens | [histone H3 N-terminal 21mer mutant T6V] N6,N6-dimethyl-L-lysine4 + succinate + formaldehyde + CO2 | - |
? | |
| 1.14.11.67 | [histone H3 N-terminal 21mer] N6,N6,N6-trimethyl-L-lysine4 + 2-oxoglutarate + O2 | H3 residue Q5 is critical for substrate recognition by KDM5A | Homo sapiens | [histone H3 N-terminal 21mer] N-terminal N6,N6-dimethyl-L-lysine4 + succinate + formaldehyde + CO2 | - |
? | |
| 1.14.11.67 | [histone H3] N6,N6,N6-trimethyl-L-lysine4 + 2-oxoglutarate + O2 | H3 residue Q5 is critical for substrate recognition by KDM5A | Homo sapiens | [histone H3] N6,N6-dimethyl-L-lysine4 + succinate + formaldehyde + CO2 | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.14.11.67 | KDM5A | - |
Homo sapiens |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.14.11.67 | 0.05 | - |
[histone H3 N-terminal 21mer mutant T6V] N6,N6,N6-trimethyl-L-lysine4 | pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
| 1.14.11.67 | 0.052 | - |
[histone H3 N-terminal 21mer] N6,N6,N6-trimethyl-L-lysine4 | pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
| 1.14.11.67 | 0.054 | - |
[histone H3 N-terminal 21mer mutant R8A] N6,N6,N6-trimethyl-L-lysine4 | pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
| 1.14.11.67 | 0.056 | - |
[histone H3 N-terminal 21mer mutant T3A] N6,N6,N6-trimethyl-L-lysine4 | pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
| 1.14.11.67 | 0.056 | - |
[histone H3 N-terminal 21mer mutant Q5A] N6,N6,N6-trimethyl-L-lysine4 | pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
| 1.14.11.67 | 0.061 | - |
[histone H3 N-terminal 21mer mutant K9A] N6,N6,N6-trimethyl-L-lysine4 | pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
| 1.14.11.67 | 0.069 | - |
[histone H3 N-terminal 21mer mutant R2A] N6,N6,N6-trimethyl-L-lysine4 | pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
| 1.14.11.67 | 0.07 | - |
[histone H3 N-terminal 21mer mutant T6S] N6,N6,N6-trimethyl-L-lysine4 | pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
| 1.14.11.67 | 0.075 | - |
[histone H3 N-terminal 21mer mutant T6A] N6,N6,N6-trimethyl-L-lysine4 | pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
| 1.14.11.67 | 0.078 | - |
[histone H3 N-terminal 18mer] N6,N6,N6-trimethyl-L-lysine4 | pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
| 1.14.11.67 | 0.101 | - |
[histone H3 N-terminal 18mer mutant K14ac/K18ac] N6,N6,N6-trimethyl-L-lysine4 | pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
| 1.14.11.67 | 0.108 | - |
[histone H3 N-terminal 18mer mutant K14A/R17A/K18A] N6,N6,N6-trimethyl-L-lysine4 | pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
| 1.14.11.67 | 0.111 | - |
[histone H3 N-terminal 13mer] N6,N6,N6-trimethyl-L-lysine4 | pH not specified in the publication, temperature not specified in the publication | Homo sapiens |
General Information
| EC Number | General Information | Comment | Organism |
|---|---|---|---|
| 1.14.11.67 | metabolism | the protein-protein interactions between KDM5A and the histone H3 tail extend beyond the amino acids proximal to the substrate mark. Deletion or mutation of residues at positions 14-18 on the H3 tail results in an 8fold increase in the KMapp value, compared to wild-type 18mer peptide. Post-translational modifications on this distal epitope can modulate KDM5A-dependent demethylation | Homo sapiens |
kcat/KM [mM/s]
| EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.14.11.67 | 0.134 | - |
[histone H3 N-terminal 21mer mutant Q5A] N6,N6,N6-trimethyl-L-lysine4 | pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
| 1.14.11.67 | 0.211 | - |
[histone H3 N-terminal 18mer mutant K14A/R17A/K18A] N6,N6,N6-trimethyl-L-lysine4 | pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
| 1.14.11.67 | 0.228 | - |
[histone H3 N-terminal 13mer] N6,N6,N6-trimethyl-L-lysine4 | pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
| 1.14.11.67 | 0.405 | - |
[histone H3 N-terminal 18mer mutant K14ac/K18ac] N6,N6,N6-trimethyl-L-lysine4 | pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
| 1.14.11.67 | 0.452 | - |
[histone H3 N-terminal 21mer mutant R2A] N6,N6,N6-trimethyl-L-lysine4 | pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
| 1.14.11.67 | 0.773 | - |
[histone H3 N-terminal 21mer mutant T6A] N6,N6,N6-trimethyl-L-lysine4 | pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
| 1.14.11.67 | 0.981 | - |
[histone H3 N-terminal 21mer mutant R8A] N6,N6,N6-trimethyl-L-lysine4 | pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
| 1.14.11.67 | 1.249 | - |
[histone H3 N-terminal 18mer] N6,N6,N6-trimethyl-L-lysine4 | pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
| 1.14.11.67 | 1.259 | - |
[histone H3 N-terminal 21mer mutant T6V] N6,N6,N6-trimethyl-L-lysine4 | pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
| 1.14.11.67 | 2.791 | - |
[histone H3 N-terminal 21mer mutant K9A] N6,N6,N6-trimethyl-L-lysine4 | pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
| 1.14.11.67 | 4.126 | - |
[histone H3 N-terminal 21mer] N6,N6,N6-trimethyl-L-lysine4 | pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
| 1.14.11.67 | 4.756 | - |
[histone H3 N-terminal 21mer mutant T6S] N6,N6,N6-trimethyl-L-lysine4 | pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
| 1.14.11.67 | 6.232 | - |
[histone H3 N-terminal 21mer mutant T3A] N6,N6,N6-trimethyl-L-lysine4 | pH not specified in the publication, temperature not specified in the publication | Homo sapiens |
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